Общество с ограниченной ответственностью "Стратегия" (RU)
发明人:
Яковлева Вера Сергеевна (RU),Николаева Зоя Калениковна (RU),Егорова Валентина Николаевна (RU)
申请号：
RU2013115658/10
公开号：
RU2013115658A
申请日:
2013.04.09
申请国别(地区):
RU
年份:
2014
代理人:
摘要：
1. A method for the preparation of interleukin-2 comprising culturing a yeast strain-producer of interleukin-2 in the culture medium for 36 to 50 hours with aeration of 0.2 to 0.8 liters of air in the mine to 100 liters of medium, obtaining a precipitate of yeast cells, mechanical disruption of the cells for from 6 minutes to 40 minutes, the precipitate of yeast purification from impurity proteins and lipids, and extracting target belka.2. The method of claim 1, wherein the yeast producer of human interleukin-2 is a strain of the yeast Saccharomyces cerevisiae, deposited in All-Union Collection of Industrial Microorganisms under No. Y-791.3. The method of claim 1, wherein culturing of the producer yeast strain interleukin-2 in the culture medium is carried out for 43 p.4. The method of claim 1, wherein the cultivation is carried out under aeration of 0.4 liter air per min per 100 liter sredy.5. The method of claim 1, wherein a mechanical cell disruption is carried out for 25 Min.6. The method of claim 1, wherein the yeast cell pellet is obtained by centrifugation or filtrovaniem.7. The method of claim 1 wherein the cells are disrupted in the presence of inhibitors proteaz.8 yeast. The method of claim 1, wherein the mechanical destruction of the cells operate using high speed disintegrator with glass sharikami.9. Interleukin-2 preparation obtained by the method according to any one pp.1-8.10. Immunomodulatory pharmaceutical preparation containing IL-2 formulation of claim 9 and at least one pharmaceutically acceptable ekstsipient.11. Pharmaceutical preparation according to claim 10, in the shape suppozitoriya.12. Pharmaceutical preparation according to claim 10, in the shape rektioly.13. Pharmaceutical preparation according to claim 10, configured in the form of capsules.1. Способ получения препарата интерлейкина-2, включающий культивирование штамма дрожжей-продуцента интерлейкина-2 в питательной среде в течение от 36 до 50 ч при аэрации от 0,2 до 0,8 л воздуха в мин
