Cranberry is a typical fruit from North America having a very high acidity that makes raw juice hardly acceptable for consumers. Electrodialysis with bipolar membrane (EDBM), an ecofriendly technology, allows the deacidification of cranberry juice and the production of pure acids without any chemical consumption due to the bipolar membrane in-situ generation of proton and hydroxyl species from water. Different levels of deacidification by electrodialysis was carried out to study the impact of the deacidification level on intestinal cell integrity. In the presence of deacidified cranberry juice, the integrity of Caco-2 cell monolayers measured by the ΔTEER was increased by 56% in comparison with raw cranberry juice.