A method of forming microorganism flora in water body and its cultivation application are disclosed, mainly related to fertilizers made of natural organic substances decomposable by microorganisms, in which a proper ratio and filter water isolation are employed to dissociate abundant minerals and microorganisms to form a microorganism flora water body, which is added to the root growth area of hydroponic cultivation crops to facilitate the microorganisms contained therein to form a symbiosis system and antagonistic microorganisms with the crop roots in the water body and generate probiotics such as microorganism flora symbiotic with gibberellin and trace elements in the water body, thereby forming an excellent natural environment beneficial to hydroponic crop growth.一種微生物菌群於水體形成方法及其耕作應用,是對應用自然界能被微生物分解的有機物質製成的肥料,在藉適當比例及濾網隔水的方式以解離出含豐富礦物質、微生物之類的微生物菌群水體,將該微生物菌群水體加入水耕種植的作物根系生長區域,以利其間的微生物能在水體中與作物根系形成共生系統、拮抗微生物,及產生激勃素與微量元素共生的微生物菌群之類的益菌在水體中,以形成良好的水耕作物生長有益的自然環境。4‧‧‧微生物菌群水體6‧‧‧水耕種植單元61‧‧‧根系生長區域62‧‧‧存放區間63‧‧‧透水膜631‧‧‧透水孔64‧‧‧覆蓋膜65‧‧‧載體8‧‧‧作物81‧‧‧根系