Provided is a method for preparing a specimen for the extensive analysis of a resident skin bacterium and/or a substance, e.g., a protein and a lipid, occurring in a horny cell layer or for the observation of the shape of a horny cell layer. A method for preparing a specimen for the analysis of a resident skin bacterium and/or a substance occurring in a horny cell layer or for the observation of the shape of a horny cell layer, said method being characterized by comprising applying a solution containing a polyvinyl alcohol-type polymer onto skin to form a thin film and then removing and collecting the formed thin film.本發明提供一種用以範圍廣泛地分析皮膚常駐菌及/或存在於角質層之蛋白質、脂質等物質、或用以觀察角質層形狀之檢體之調製方法。 本發明係一種檢體之調製方法，其特徵在於：其係調製用以分析皮膚常駐菌及/或存在於角質層之物質、或觀察角質層形狀之檢體之方法，且將含有聚乙烯醇系聚合物之溶液以形成薄膜之方式塗抹在皮膚上並剝離回收所形成之薄膜。
