The present invention relates to the gamma delta T cells of engineering and use the gamma delta T cells as the method for therapeutic agent, the therapeutic agent has the effective and selection sexuality for targeting selected antigen. The gamma delta T cells of the engineering of the disclosure are suitable for treating various cancers, infectious diseases and immune disorders. Method for the gamma delta T cells group of engineering and non-engineering to be expanded to the upper useful amount for the treatment of is also disclosed. The gamma delta T cells of the engineering of the disclosure can be universal donor, and can be administered to the subject with any MHC haplotypes.
