[Problem] To enable production of high quality eggs in poultry, and to provide a method for improving production thereof. [Solution] A method for producing a hen egg having improved quality and productivity, the method characterized in comprising a step for feeding to a hen a supplemented feed obtained by adding to poultry feed an immunostimulating substance produced by cytolysis that accompanies sporulation of MRE symbiotic bacteria and/or by feeding supplemented drinking water obtained by adding the immunostimulating substance to drinking water; wherein the immunostimulating substance is obtained by culturing the MRE symbiotic bacteria, leaving the resulting culture fluid in a depleted state to thereby cause the symbiotic bacteria to convert to endospores, and removing impurities, including the endosporic bacteria, from the culture solution.