The present invention discloses a method for breeding Brassica napus varieties and materials with a double haploid induction line of rapeseed, including: 1) determining target traits of breeding restorer lines, maintainer lines and conventional varieties of Brassica napus； 2) crossing or convergently crossing two or more Brassica napus with the target traits； 3) pollinating the cross or back-cross progenies with the double haploid induction line of rapeseed； 4) identifying the stability of the induced progenies； 5) performing test-cross identification or yield and resistance identification on the stable progenies； and 6) forming stable restorer lines and maintainer lines for cross breeding combination or for forming conventional varieties. The present invention can quickly and efficiently obtain rapeseed materials or conventional varieties with application value in breeding on a large scale, and lays a solid foundation for genetic breeding of Brassica napus, innovation of breeding resources, breeding of conventional rapeseed varieties, and breeding of new varieties of hybrid rapeseed. The method of the present invention can greatly improve the breeding speed and efficiency of hybrid or conventional varieties of Brassica napus, and reduce the human and material resources.
