The present invention provides a composition for activating activity of immune cells comprising a mushroom component, a beneficial bacterial component and an excipient. The present invention also provides a method for preparing the above composition comprising culturing mushroom mycelia in liquid to obtain the mushroom component, culturing the beneficial bacteria in suitable medium to obtain the beneficial bacterial component, and mixing the mushroom component, the beneficial bacterial component and an excipient to form the composition.本發明係提供一種活化動物體內免疫細胞活性的組成物,包括菇蕈成分,益菌成分及賦形劑。本發明亦提供該組成物的製備方法,包括:取菇蕈菌絲體進行液態培養,以獲得菇蕈成分;以適當培養基培養益菌,以獲得益菌成分;及混合所得菇蕈成分、益菌成分與賦形劑,以獲得該組成物。