Bradley Didion,Cindy L. Ludwig,David R. Helbling,Gary P. Vandre,Gary Durack,Jeffrey A. Graham,Jeffrey D. Wallace,Jeremy T. Hatcher,KATHLEEN S. CROWLEY,Lon A. Westfall,Muhammad Anzar,Niraj V. Nayak,BRADLEY DIDION,CINDY L LUDWIG,DAVID R HELBLING,GARY P VANDRE
申请号：
BRPI0408857
公开号：
BRPI0408857B1
申请日:
2004.03.29
申请国别(地区):
BR
年份:
2018
代理人:
摘要：
"Apparatus, methods and processes for separating particles and for providing sex-separated animal sperm." apparatus and methods for analyzing particles, including apparatus and methods for a sperm separation process including: collecting sperm from an animal (30)； the selection of dyeing conditions (47a)； dyeing the sperm with dna selective fluorescent dye (48)； sperm cell separation according to sex chromosome content (55)； and cryopreservation of a separate sperm population (61) until used for artificial insemination. One embodiment includes apparatus (1001) and methods for using a plurality of flow cytometry units (9) that share an integrated platform for separating sperm cells. In one embodiment, cytometric flow separation includes the use of the following apparatus and methods: an orientation nozzle having a deflector (101)； an optical epi-lighting system (109)； split scanning of DNA regions located within cell nuclei (225)； digital signal processing, including synchronous sampling of analog output signals (701), pulse waveform feature extraction (497) from an approximation of a first order derivative of a pulse waveform (497) ) at one point on the wrist any of the various separation strategies； and an automated separation calibration system (4201). In one embodiment, digital signal processing includes sampling analog output signals 701 sometimes relative to the emission of pulses originating from an illumination laser. other embodiments are substantially different from the foregoing, including individual step-directed embodiments or systems that may be used for any of a variety of particle analysis applications."aparelho, métodos e processos para separar partículas e para prover esperma de animal separado por sexo". aparelho e métodos para analisar partículas, incluindo aparelho e métodos para um processo de separação de esperma incluindo: a coleta de esperma de um animal (30)； a seleção das condições de tingimento (47a)； o tingimento do esperma com corante fl
