Provided herein is a fluid pathway connection assembly, e.g., for connecting a fluid flowpath within a drug delivery device for delivery of a medicament to a target site. The fluid pathway connection assembly comprises a hollow needle piercing member, a sleeve fixedly engaged with the piercing member and a pierceable seal configured to sealingly engage the piercing member or the sleeve. Upon, sealing engagement, the piercing member or the sleeve and the pierceable seal form a volume within which a portion of the piercing member is disposed. The fluid pathway connection assembly is activated by relative movement between the piercing member and the pierceable seal and activation causes the piercing member to pierce the pierceable seal, thereby establishing a fluid flowpath through the pierceable seal. Upon incorporation of the assembly into a sealed fluid flowpath, the volume is isolated from the environment and maintains the sterile condition of the portion of the piercing member disposed therein. Also provided herein are drug delivery pumps and methods of operating and assembling the assemblies and pumps described herein.在此提供一種例如用於連接藥物遞送裝置內的流體流動路徑以用於將藥物遞送到目標部位的流體路徑連接組件。該流體路徑連接組件包括中空針刺穿構件、與該刺穿構件固定地接合的套筒和被配置成密封地接合該刺穿構件或該套筒的可刺穿密封件。在密封接合時,該刺穿構件或該套筒和該可刺穿密封件形成其中設置有該刺穿構件的一部分的容積。該流體路徑連接組件藉由該刺穿構件與該可刺穿密封件之間的相對移動而被啟動,並且啟動引起該刺穿構件刺穿該可刺穿密封件,從而建立通過該可刺穿密封件的流體流動路徑。在將該組件併入密封的流體流動路徑中時,該容積與環境隔離並且維持其中設置的該刺穿構件的該部分的無菌狀態。在此還提供藥物遞送泵以及操作和組裝在此描述的該等組件和泵的方法。