The present invention discloses a method for preparing sericin hydrogel. The method comprises weighing silkworm silkworm fibroin-deficient mutant varieties, extracting them with an aqueous LiBr or LiCl solution, dialysis and purification to obtain a non-degraded mass percentage concentration of 0.1 to 4%. After the sericin aqueous solution was obtained, the sericin aqueous solution was concentrated to 1.5 to 10.0%, and a crosslinking agent was added to the concentrated sericin aqueous solution (crosslinking agent 2 to 500 μL with respect to 1 mL of the sericin aqueous solution). And mixed at 4 to 45 ° C. for 5 seconds to 36 hours to obtain a hydrogel. The hydrogel has bioactivity and versatility, and as a growth factor, drug and cell carrier, various soft tissue injuries including but not limited to skin injury, muscle injury, vascular injury, nerve injury, myocardial injury, etc. It can be used for repairing and treating diseases. [Selection] Figure 11【課題】本発明は、セリシンハイドロゲルの調製方法を開示する。【解決手段】該方法は、カイコシルクフィブロイン欠失型突然変異品種の蚕繭を量り取り、ＬｉＢｒまたはＬｉＣｌ水溶液によって抽出し、透析、純化によって、分解されていない質量百分率濃度０．１～４％のセリシン水溶液を得た後、セリシン水溶液を濃縮して１．５～１０．０％とし、濃縮後のセリシン水溶液に対して架橋剤を加え（セリシン水溶液１ｍＬに対し架橋剤２～５００μＬ）、十分に混合した後、４～４５℃で５秒～３６時間置き、ハイドロゲルを得る。該ハイドロゲルは、生体活性と多用途性を備え、成長因子、薬物および細胞の担体として、皮膚損傷、筋肉損傷、血管損傷、神経損傷、心筋損傷等を含むがこれらに限定されない多種の軟組織損傷の修復及び疾病の治療に使用できる。【選択図】図１１
