米満 吉和,原田 結,寺石 紘司,YONEMITSU YOSHIKAZU,HARADA YUI,TERAISHI KOJI
申请号：
JP2020067164
公开号：
JP2020108405A
申请日:
2020.04.03
申请国别(地区):
JP
年份:
2020
代理人:
摘要：
PROBLEM TO BE SOLVED: To provide a population of NK cells having higher cytotoxic activity. Provided is a method for producing a population of NK cells, which comprises the steps of: (1) obtaining a mononuclear cell population containing NK cells； (2) the obtained mononuclear cell population. From (3), (3) culturing the remaining cell population from which CD3 positive cells have been removed to obtain a population of cultured NK cells, (4) the obtained population of NK cells Mixing with the antibody drug to obtain a population of NK cells to which the antibody drug is bound. [Selection diagram] Fig. 6【課題】細胞傷害活性がより高いＮＫ細胞の集団を提供する。【解決手段】以下の工程を含む、ＮＫ細胞の集団の製造方法を提供する：（１）ＮＫ細胞を含む単核球の細胞集団を得る工程、 （２）得られた単核球の細胞集団から、ＣＤ３陽性細胞を除去する工程、（３）ＣＤ３陽性細胞が除去された残りの細胞集団を培養し、培養されたＮＫ細胞の集団を得る工程、（４）得られたＮＫ細胞の集団と抗体医薬とを混合し、抗体医薬が結合したＮＫ細胞の集団を得る工程。【選択図】図６
