Described is a method of producing a storage stable autologous composition comprising regenerative growth factors and cytokines obtained from platelets. The method comprises the steps of collecting or providing blood from a mammal； delivering the blood to a receptacle； centrifuging the blood to separate a platelet rich plasma component from the blood； collecting the platelet rich plasma component； passing the platelet rich plasma component through a small pore filter to produce the storage stable autologous composition comprising regenerative growth factors and cytokines derived from platelets； and collecting the storage stable autologous composition. The storage stable autologous composition retains regenerative biological activity for up to at least nine months when stored in a frozen state. Also described is a storage stable autologous composition comprising regenerative growth factors and cytokines obtained from platelets produced by the method as described.
