A transplanting device (11) separates a seedling bed piece (14) with a seedling (N) from a seedling bed (12) along slits (22) and transplants the separated seedling bed piece (14) to a permanent planting panel (16). The transplanting device (11) includes a separating mechanism (15) and a transplanting mechanism (18). The separating mechanism (15) includes a holding member (30) capable of holding the seedling bed piece (14). The separating mechanism (15) is capable of separating the seedling bed piece (14) from the seedling bed (12) by moving the holding member (30) and the seedling bed (12) relative to each other with the holding member (30) holding the seedling bed piece (14). The transplanting mechanism (18) is capable of transplanting the separated seedling bed piece (14) to the permanent planting panel (16). The separating mechanism (15) includes an engagement bar (42) capable of hampering movement of an adjacent seedling bed piece (14B) adjacent to a holding-target seedling bed piece (14A) held by the ho