OHIO UNIVERSITY;KIELISZEWSKI, MARCIA;XU, JIANFENG;COOPER, IVER P.
发明人:
KIELISZEWSKI, MARCIA,XU, JIANFENG,COOPER, IVER P.
申请号：
WO2007US73137
公开号：
WO2008008766(A2)
申请日:
2007.07.10
申请国别(地区):
世界知识产权组织国际局
年份:
2008
代理人:
摘要：
Plant cells contain particular proline hydroxylases whose specificity determines which prolines, in a protein expressed therein, are hydroxylated. The resulting hydroxyprolines, in turn, my be glycosylated. It is the presence of glycosylated hydroxyprolines which is the most important determinant of the degree of secretion of the protein. One may modify the proline hydroxylation capabilities of the plant cell by co-expressing in that cell at least one exogeneous proline hyrdoxylase which will hydroxylate at least one proline site in the protein which is not hydroxylated by the endogeneous proline hydroxylase, but which, if hydroxylated, can than be glycosylated by the plant cell, resulting in increased Hyp-glycosylation. Moreover, even if the co-expressed exogenous proline hydroxylase doesn't achieve the desired level of Hyp-glycosylation in the native protein, such co-expression may still be used in conjunction with modification of the protein itself to increase the number and/or the likelihood of Hyp-glycosylation sites.
