Provided is a sheet-shaped cell culture containing cardiomyocytes derived from pluripotent stem cells having excellent functional properties. A sheet-like cell culture obtained by culturing a cell population containing cardiomyocytes derived from pluripotent stem cells, wherein the ratio of the number of cardiomyocytes derived from pluripotent stem cells to the total number of cells in the cell population is 50% -70% of the sheet-shaped cell culture, a method for producing the sheet-shaped cell culture, a composition containing the sheet-shaped cell culture, the sheet-shaped cell culture or treatment of a disease using the composition Method.機能的特性に優れた、多能性幹細胞由来の心筋細胞を含むシート状細胞培養物を提供する。 多能性幹細胞由来の心筋細胞を含む細胞集団を培養して得られるシート状細胞培養物であって、前記細胞集団の全細胞数に対する多能性幹細胞由来の心筋細胞数の割合が、50%~70%である、前記シート状細胞培養物、該シート状細胞培養物の製造方法、該シート状細胞培養物を含む組成物、該シート状細胞培養物または該組成物を用いた疾患の処置方法。