PETRE MARIAN,PETRE MARIAN,TEODORESCU ALEXANDRU,TEODORESCU ALEXANDRU
申请号：
RO201001049
公开号：
RO126279B1
申请日:
2010.11.03
申请国别(地区):
RO
年份:
2013
代理人:
摘要：
The invention relates to a biotechnological process for in vitro cultivation of macromycetes of the species Lentinus edodes and to the fungal biomass of the species Lentinus edodes, to be applied in food industry and in therapy. According to the invention, the process provides for cultivation of macromycetes of the species Lentinus edodes on a liquid nutritive medium, followed by transfer thereof into cultivation vessels, sterilization at 121°C for 15 min, cooling at temperatures of 25°C, aseptic inoculation with pure culture mycelium, incubation at a constant temperature of 23...25°C and a stirring rate of 100...150 rpm, maintaining under submerged fermentation condition for 5...10 days, to obtain a white fungal biomass which is separated from the culture medium by filtration and the resulting sediment is subjected to mortar trituration, is slowly dehydrated in a warm air current, at a temperature of 30...35°C and is conditioned as granules of 0.1...0.5 mm.
