A pH-modified luminescent composition and methods. In composition, a marker exhibiting luminescence change over a indicatory period. pH-based luminesce change may be in intensity or in wavelength. One embodiment provides a luminescent composition, including a selected luminescent marker having a first phase and a selected ionizing agent comprising a second phase. The selected luminescent marker exhibits luminescence for an indicatory period responsive to intermixing of the first phase with the second phase. A selected antimicrobial agent combined with the first phase of the luminescent composition, wherein intermixing of the first phase with the second phase is at least a portion of a hand hygiene protocol. A method embodiment of using the luminescent composition, can include providing a luminescent marker providing a ionizing agent intermixing the luminescent marker with the ionizing agent causing a change in pH in the selected luminescent marker and exhibiting luminescence for a predetermined indicatory period.
