PROBLEM TO BE SOLVED: To provide a method for efficiently producing a cutting seedling in which rooting rate in a cutting scion of a plant that accumulates secondary metabolite can be increased.SOLUTION: A method for producing a cutting seedling of a plant that accumulates secondary metabolite includes a step of letting a cutting scion root under a condition of 120-270 &mumol/m/sec of light intensity and 60% Rh or more of humidity. It is preferable that: carbonic acid gas concentration in the step is 500-1500 ppm the plant is a medicinal plant and the step is conducted in a culture vessel.