PURPOSE: A conditioning shampoo composition containing biosurfactant is provided to maximize the formation of coacervate and ensure hair conditioning effect. CONSTITUTION: A conditioning shampoo composition contains biosurfactant, glyceryl quaternary ammonium cation surfactant of the chemical formula 1 and cationic polymer as active ingredient. In the chemical formula 1, R1 is alkyl group of 12-22 carbon atoms, branched alkyl group or alkenyl group, R2 and R3 are independently alkyl group of 1-3 carbon atoms; and R4 is (CH2CHOHCH2O)nH. The biosurfactant is glycolipid, lipopeptide, lipotprotein, phospholipid, sugar esters or polymer biosurfactant.본 발명은 바이오계면활성제 함유 컨디셔닝 샴푸 조성물에 관한 것으로, 바이오계면활성제와, 글리세릴 4급 알킬 암모늄 양이온 계면활성제, 및 양이온 폴리머를 동시에 포함시킴으로써 사용감이 우수한 코아세르베이트를 생성시키고, 또한 이러한 코아세르베이트 형성을 극대화하여 컨디셔닝 효과를 강화한 컨디셔닝 샴푸 조성물에 관한 것이다.