The present invention discloses an in vitro method to provide biological t issue with a heparin coating comprising the following steps； linking a biotin reagent to biological tissue, linking an avidin reagent to the biotinylated biological tissue, and linking a heparin reagent to the formed layer of avidin on the biological t issue thus forming a heparin coating. The invention further discloses a heparin coating on biological t issue, the use of a heparin coating, and the biological t issue coated with a heparin layer according to the method.
