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Screening method for selecting plants that show a reduced wound-induced surface discolouration
专利权人:
RIJK ZWAAN ZAADTEELT EN ZAADHANDEL B.V.
发明人:
VAN DUN, CORNELIS MARIA PETRUS
申请号:
NZ56957907
公开号:
NZ569579A
申请日:
2007.01.08
申请国别(地区):
NZ
年份:
2013
代理人:
摘要:
Provided is a method for screening a population of plants or mushrooms, or parts thereof for the presence therein of individuals that show a reduced discolouration as compared to a control plant, mushroom or part thereof, which method comprises a) providing a population of plants, mushrooms or parts thereof from the population; b) creating a wound surface on the plants, mushrooms or parts thereof; c) incubating the plant, mushrooms or parts thereof or the wound surfaces created thereon in an aqueous environment or with a colouring substrate to allow for discoloration to occur therein or thereon; d) observing the wound-induced discolouration in or on the plants, mushrooms or parts thereof; e) comparing the observed discolouration with the discolouration that is observed in the control plant, mushroom or part thereof to identify plants, mushrooms or parts thereof that show no discolouration or a discolouration that is reduced as compared to the control plant, mushroom or part thereof, wherein the plants or mushrooms, or parts thereof used in the screen are of a developmental stage different from, or a tissue other than the stage or tissue in which discolouration occurs; and f) selecting the plants, mushrooms or parts thereof that show no discolouration or a discolouration that is reduced as compared to the control plant, mushroom or parts thereof. The colouring sensitive substrate can be chlorogenic acid, catechol, caffeic acid and DOPA, among others.
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中国工程科技知识中心
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