The present invention relates to a sterilizer capable of removing moisture using vacuum. The sterilizer includes: a sterilization chamber formed to seal a sterilization space in which an object to be sterilized is contained; a chamber suction line connected to the sterilization chamber; a vacuum pump installed on the chamber suction line for vacuum suction; a suction device suction line installed to make the vacuum pump perform vacuum suction; a suction device main body which is connected with an upper part of the suction device suction line, maintains airtightness of the inside thereof, and collects moisture; a suction deice vacuum control valve installed on the suction device suction line to open and close the suction device suction line; a suction device nozzle which is connected to an upper part of the suction device main body and sucks external air with moisture into the suction device main body by vacuum suction; and a nozzle suction control valve installed to open and close the suction device nozzle. According to the present invention, the vacuum pump, providing a vacuum condition to the sterilization chamber, is used to easily remove moisture from the object to be sterilized such as a medical tool requiring moisture removal. Therefore, time and effort to remove moisture are reduced, and thus medical tools can be easily and rapidly sterilized. The vacuum pump is prevented from being damaged by moisture, and thus the sterilizer can have excellent durability. The sterilizer does not additionally require a compressor or a suction device, and can prevent incomplete sterilization, which is caused by moisture in the sterilized object after washing the object.본 발명은 멸균대상물이 멸균을 위해 수용되는 멸균공간이 밀폐되도록 형성되는 멸균챔버; 상기 멸균챔버에 연결되는 챔버흡입라인; 상기 챔버흡입라인에 진공 흡입을 위해 설치되는 진공펌프; 상기 진공펌프가 진공 흡입하도록 설치되는 흡입기흡입라인; 상기 흡입기흡입라인이 상부에 연결되고, 내부가 기밀을 유지하며, 수분이 수집되는 흡입기본체; 상기 흡입기흡입라인에 개폐를 위해 설치되는 흡입기진공제어밸브; 상기 흡입기본체의 상부에 연결되고, 상기 흡입기흡입라인을 통해서 공급되는 진공 흡입에 의해 상기 흡입기본체로 외부의 에어가 수분과 함께 흡입되도록 하는 흡입기노즐;