HANSEO UNIVERSITY ACADEMIC COOPERATION FOUNDATION;장종화;한서대학교 산학협력단;JANG, JONG HWA
发明人:
JANG, JONG HWA,장종화,OH, TAE JIN,오태진,CHO, JEON HO,조전호
申请号：
KR1020120020414
公开号：
KR1020130098692A
申请日:
2012.02.28
申请国别(地区):
KR
年份:
2013
代理人:
摘要：
PURPOSE: A method for isolating pilocarpine is provided to maximize the quantity of pilocarpine according to the combination of enzymes, temperature, and pH concentration. CONSTITUTION: A method for isolating pilocarpine comprises the steps of: pulverizing dried jaborandi leaves； mixing three times weight of distilled water with the pulverized jaborandi and preparing a mixture solution； adjusting the mixture solution to pH 3； heating the mixture solution at 100°；C for one hour； adjusting the heated solution to pH 10.5； mixing the solution with twice weight of chloroform and inducing phase separation； isolating and concentrating a lower liquid from the solution； mixing the concentrated lower liquid with twice weight of sulfuric acid and inducing phase separation； isolating and adjusting a supernatant to pH 10.5； mixing the supernatant with twice weight of chloroform and inducing phase separation； isolating and concentrating a lower liquid； and adding a mixture of acetone and methanol to the concentrated lower liquid and extracting pilocarpine. [Reference numerals] (AA) Pulverizing jaborandi leaves； (BB) Mixing distilled water with the pulverized jaborandi leaves； (CC) Adjusting the mixture solution to pH 3； (DD) Heating the mixture solution at 100°；C for one hour； (EE) Mixing the solution with chloroform and isolating a lower liquid from the solution； (FF) Concentrating the lower liquid； (GG) Concentrating the lower liquid/extracting pilocarpine； (HH) Adjusting a upper liquid to pH 10.5； (II) Isolating the upper liquid； (JJ) Mixing with sulfuric acid and separating phases본 발명은 필로카르핀 추출방법에 관한 것으로, 보다 상세하게는 추출공정 중 온도, pH 및 분해효소 조합에 따라 추출되는 필로카르핀 양을 극대화할 수 있는 필로카르핀 추출방법에 관한 것이다.
