БАДЕР Мартин (DE),БЕНЕР Моника (DE),ГРОССМАНН Адельберт (DE),МОЛЬ Зильке (CH)
申请号：
RU2013111678/15
公开号：
RU2013111678A
申请日:
2011.08.25
申请国别(地区):
RU
年份:
2014
代理人:
摘要：
1. A process for preparing lipid particles comprising the following steps: i) providing a first solution comprising a denatured protein, ii) adding the first solution to second solution comprising at least one lipid and detergent but does not contain protein, and iii) removing detergent from the solution obtained in step ii), to obtain a lipid chastitsy.2. A method according to claim 1, characterized in that the second solution has a volume of from about 3 to about 20 volumes of the first rastvora.3. A method according to claim 1, characterized in that the first solution is free from lipidov.4. A method according to claim 1, characterized in that said protein has an amino acid sequence selected from the following amino acid sequences: SEQ ID NO: 01, 02, 04-52, 66 or 67 or comprises at least a continuous fragment thereof comprising at least 80% amino acid sequence of SEQ ID NO: 01, 02, 04-52, 66 or 67.5. A method according to claim 4, characterized in that said protein is a tetranektin apolipoprotein-AI, which has the amino acid sequence of SEQ ID NO: 01, or SEQ ID NO: 02, or SEQ ID NO: 66, or SEQ ID NO: 67.6. A method according to claim 1, characterized in that at least one lipid comprises two different fosfatidilholina.7. A method according to claim 6, characterized in that the first phosphatidylcholine is POPC, and the second is a phosphatidylcholine DPPC.8. A method according to claim 1, characterized in that said detergent is selected from the group consisting of cholic acid, Zwittergent or sol.9. A method according to claim 1, characterized in that the method comprises, after step ii) and before step iii) following step iia) incubating the solution obtained in ii) .10 step. A method according to claim 1, wherein Th1. Способ получения липидной частицы, включающий следующие стадии:i) получение первого раствора, включающего денатурированный белок,ii) добавление первого раствора ко второму раствору, содержащему по меньшей мере один липид и детергент, но не содержащ
