RAO, GALLA CHANDRA,LARSON, CHRISTOPHER,REPOLLET, MADELINE,RUTNER, HERMAN,TERSTAPPEN, LEON,O'HARA, SHAWN MARK,GROSS, STEVEN
申请号：
CA2457894
公开号：
CA2457894C
申请日:
2002.08.23
申请国别(地区):
CA
年份:
2014
代理人:
摘要：
The methods and reagents described in this invention are used to analyzecirculating tumor cells, clusters, fragments, and debris. Analysis isperformed with a number of platforms, including flow cytometry and theCellSpotter®* fluorescent microscopy imaging system. Analyzing damagedcells has shown to be important. However, there are two sources of damage: invivo and in vitro. Damage in vivo occurs by apoptosis, necrosis, or immuneresponse. Damage in vitro occurs during sample acquisition, handling,transport, processing, or analysis. It is therefore desirable to confine,reduce, eliminate, or at least qualify in vitro damage to prevent it frominterfering in analysis. Described herein are methods to diagnose, monitor,and screen disease based on circulating rare cells, including malignancy asdetermined by CTC, clusters, fragments, and debris. Also provided are kits forassaying biological specimens using these methods.
