Francisco MARTÍN MOLINA,Karim BENABDELLAH ELKHLANJI,Marién COBO PULIDO,Pilar MUÑOZ FERNÁNDEZ
申请号：
US16347530
公开号：
US20200199619A1
申请日:
2017.11.03
申请国别(地区):
US
年份:
2020
代理人:
摘要：
In the present invention, the inventors have generated a Tet-On all-in-one construct that tightly regulate transgene expression in human stem cells using the original TetR repressor. By using appropriate promoter combinations and shielding the integrative construct with the Is2 insulator, they have constructed the Lent-On-Plus Tet-On system that achieves efficient transgene regulation in human multipotent and pluripotent stem cells. The generation of inducible stem cell lines with the Lent-ON-Plus system did not require selection or cloning, and transgene regulation is maintained after long-term cultured and upon differentiation toward different lineages. The present invention thus offers a solution to the need to improve transgene expression systems in stem cells.
