PURPOSE: A cell culture medium is provided to replace a commercial culture medium using an infusion solution and an injection solution which are approved as a pharmaceutical product.CONSTITUTION: A method for manufacturing a cell culture medium comprises the step of mixing an infusion solution or an injection solution for supplying vitamin an infusion solution or an injection solution for supplying amino acids and an infusion solution or an injection solution for supplying inorganic salts. The cell culture medium has the similar composition as a commercial cell culture medium. The method further comprises the step of adding glucose, inorganic salts supplements, antibiotics, and antifungal agents.COPYRIGHT KIPO 2013본 발명은 세포 배양액에 관한 것으로, 더욱 상세하게는 세포치료제 등을 위한 안전하고, 안정되며 유효한 세포배양액에 관한 것이다.