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PRODUCTION METHOD FOR RETINAL TISSUE
专利权人:
发明人:
Atsushi KUWAHARA,Suguru YAMASAKI,Yasushi HIRAMINE,Yoshiki SASAI,Masayo TAKAHASHI
申请号:
US15521387
公开号:
US20170313981A1
申请日:
2015.10.23
申请国别(地区):
US
年份:
2017
代理人:
摘要:
The present invention provides a method for producing retinal cells or a retinal tissue, comprising the following steps (1)-(3):(1) a first step of culturing human pluripotent stem cells in the absence of feeder cells and in a medium comprising a factor for maintaining undifferentiated state,(2) a second step of culturing the pluripotent stem cells obtained in the first step in suspension in the presence of a Sonic hedgehog signal transduction pathway activating substance to form a cell aggregate, and(3) a third step of culturing the aggregate obtained in the second step in suspension in the presence of a 1) a BMP signal transduction pathway activating substance to obtain an aggregate containing retinal cells or a retinal tissue.
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