PROBLEM TO BE SOLVED: To provide methods for producing a composition having a cell-killing activity, and to provide methods for producing a pharmaceutical composition for treating cancer, which contains the composition.SOLUTION: Provided are a composition having a cell-killing activity, which is produced by a method comprising: culturing malignant tumor-derived cells in a culture medium at least until the density of cells is a density that does not hinder passage； replacing the culture medium with a physiological buffered saline after culturing； and collecting the physiological buffered saline after the time when morphological death of the malignant tumor-derived cells is observed, and use of the composition for producing medicaments.SELECTED DRAWING: NoneCOPYRIGHT: (C)2020,JPO&INPIT【課題】殺細胞活性を有する組成物の製造方法、および該組成物を含むがん治療用医薬組成物の製造方法の提供。【解決手段】悪性腫瘍由来細胞を少なくとも細胞の密度が継代に支障のない密度になるまで培養培地中で培養すること、培養後培地を生理的緩衝塩類溶液と交換すること、該悪性腫瘍由来細胞の形態学的な死滅が観察される時期後に生理的緩衝塩類溶液を回収すること、を含む方法で製造される殺細胞活性を有する組成物、および該組成物の医薬製造のための使用。【選択図】なし
