KIM, SEUNG HYUK,김승혁,KIM, YOUNG SIK,김영식,LEE, SEONG BAEK,이성백,JANG, JU WOONG,장주웅,SHIM, YOUNG BOCK,심영복
申请号：
KR1020120013434
公开号：
KR1012124990000B1
申请日:
2012.02.09
申请国别(地区):
KR
年份:
2012
代理人:
摘要：
PURPOSE: A method for purifying BMP especially, BMP7 is provided to simplify the process and to enhance BMP final yield. CONSTITUTION: A method for purifying BMP 7 comprises a step of applying affinity chromatography using sulfate resin in a solution containing BMP 7. The affinity chromatography comprises: a step of equilibrating a column with a buffer solution containing Tris； a step of loading the solution containing BMP7 onto the column； a step of washing the column with a buffer solution containing Tris and arginine； and a step of eluting the column with the buffer solution.본 발명은 BMP, 특히 BMP7의 정제 방법에 관한 것이다. 또한, 본 발명은 상기 정제 방법에 따라 고순도 및 고회수율로 정제된 BMP, 바람직하게는 BMP7에 관한 것이다.
