Process for the preparation of purified daptomycin, daptomycin purification method, the composition of daptomycin, the use of the pharmaceutical composition and the insulated compound
KELLEHER THOMAS J.,KELLEHER THOMAS J.,LAI JAN-JI,LAI JAN-JI,DECOURCEY JOSEPH P.,DECOURCEY JOSEPH P.,LYNCH PAUL D.,LYNCH PAUL D.,ZENONI MAURIZIO,ZENONI MAURIZIO,TAGLIANI AURO R.,TAGLIANI AURO R.
申请号：
PL40057501
公开号：
PL400575A1
申请日:
2001.01.18
申请国别(地区):
PL
年份:
2013
代理人:
摘要：
The invention discloses highly purified daptomycin and to pharmaceutical compositions comprising this compound. The invention discloses a method of purifying daptomycin comprising the sequential steps of anion exchange chromatography, hydrophobic interaction chromatography and anion exchange chromatography. The invention also discloses a method of purifying daptomycin by modified buffer enhanced anion exchange chromatography. The invention also discloses an improved method for producing daptomycin by fermentation of Streptomyces roseosporus. The invention also discloses high pressure liquid chromatography methods for analysis of daptomycin purity. The invention also discloses lipopeptide micelles and methods of making the micelles. The invention also discloses methods of using lipopeptide micelles for purifying lipopeptide antibiotics, such as daptomycin. The invention also discloses using lipopeptide micelles therapeutically.
