A hemoglobin and hematocrit analyzer, and an analyzing method thereof include: scanning and taking an image signal of the palpebral conjunctiva of a subject by a scanning unit receiving the image signal by an analyzing unit connected to the scanning unit providing a default colorimetric scale by a database connected to the analyzing unit inputting a clinical testing result into the analyzing unit through a input unit connected to the analyzing unit. Wherein, the image signal is transformed by the analyzing unit to a measuring color value, and the measuring color value is compared with the default colorimetric scale to obtain a test result and feedback of the measuring color value and the clinical testing result is provided into the database.本發明提供一種血色素及血球比容的檢測系統及方法,其包含:利用掃描單元掃描受試者之下眼瞼結膜,並擷取受試者之下眼瞼結膜之影像訊號;透過與掃描單元連接,分析單元接受該影像訊號;資料庫與分析單元連接,並提供預設比色表;以及藉由輸入單元連接分析單元並輸入受試者之臨床檢測結果。其中分析單元將影像訊號轉換為量測色彩值,並將量測色彩值與預設比色表比較以取得檢測結果;以及分析單元反饋量測色彩值及臨床檢測結果至資料庫。10‧‧‧檢測系統100‧‧‧掃描單元110‧‧‧攝像單元111‧‧‧影像訊號120‧‧‧分析單元121‧‧‧檢測結果130‧‧‧資料庫131‧‧‧預設比色表140‧‧‧輸入單元141‧‧‧臨床檢測結果