Kumar, Sandeep,Alabed, Diaa,Bennett, Sara,Gupta, Manju,Jayne, Susan,Wright, Terry
申请号:
AU2012363063
公开号:
AU2012363063B2
申请日:
2012.11.12
申请国别(地区):
AU
年份:
2018
代理人:
摘要:
Provided are constructs and methods for expressing multiple genes in plant cells and/or plant tissues. The constructs provided comprise at least one bi directional promoter linked to multiple gene expression cassettes, wherein the bi directional promoter comprises a functional promoter nucleotide sequence from Sugar Cane Bacilliform Virus promoter. In some embodiments, the constructs and methods provided employs a bi directional promoter based on a minimal core promoter element from a Zea mays Ubiquitin 1 gene, or a functional equivalent thereof, and nucleotide sequence elements from a Sugar Cane Bacilliform Virus promoter. In some embodiments, the constructs and methods provided allow expression of genes between three and twenty.