KUMAR, SANDEEP,ALABED, DIAA,BENNETT, SARA,GUPTA, MANJU,JAYNE, SUSAN,WRIGHT, TERRY
申请号:
EP12862691
公开号:
EP2797407A4
申请日:
2012.11.12
申请国别(地区):
EP
年份:
2015
代理人:
摘要:
Provided are constructs and methods for expressing multiple genes in plant cells and/or plant tissues. The constructs provided comprise at least one bi-directional promoter linked to multiple gene expression cassettes, wherein the bi-directional promoter comprises a functional promoter nucleotide sequence from Sugar Cane Bacilliform Virus promoter. In some embodiments, the constructs and methods provided employs a bi-directional promoter based on a minimal core promoter element from a Zea mays Ubiquitin-1 gene, or a functional equivalent thereof, and nucleotide sequence elements from a Sugar Cane Bacilliform Virus promoter. In some embodiments, the constructs and methods provided allow expression of genes between three and twenty.