The present invention relates to a protaetiamycine antibiotic peptide derivative with high antibacterial activities against multidrug resistant bacteria and its use, and more specifically, to a protaetiamycine antibiotic peptide derivative derived from Protaetia brevitarsis seulensis larva and its use as antibacterial agent against multidrug resistant bacteria. According to one embodiment of the present invention, provided is an antimicrobial peptide with amino acid sequence of SEQ ID NO: 1.본 발명은 흰점박이 꽃무지유충으로부터 분리된 프로태티아마이신 항균펩타이드 유도체 및 그의 다제내성균에 대한 항균제로서의 용도에 관한 것이다.