A fungus (A) that can produce cholesterol (I) autonomously, is new. Independent claims are also included for: (1) production of (I) by culturing (A); (2) production of (I) or its metabolic intermediates or a mixture of sterols labeled by 13C or 14C, by culturing (A); (3) production of an isotopic mixture of (I), its intermediates or metabolites labeled at different positions by isotopic markers, by culturing (A), and (4) labeled (I), its intermediates or metabolites, prepared by method (3).La présente invention concerne la production de cholestérol chez des organismes du règne des Fungi. Plus particulièrement, l'invention concerne des Fungus génétiquement modifiés produisant de manière autonome du cholestérol à partir d'une source de carbone simple. L'invention concerne également l'utilisation de Fungus selon l'invention pour la production de cholestérol non-marqué et marqué.