A clip pushing mechanism used in a surgical clip applicator for loading and pushing clips that are arranged in series with a clip pitch defined between each two adjacent said clips is disclosed to include a clip holder bar including an accommodation channel for accommodating the clip in a series, a clip feeding ladder member including a clip pushing portion and a plurality of driven portions spaced along the length thereof with a pitch defined between each two adjacent driven portions, and a clip pusher including a body, a front pusher for pushing the leading clip and a rear pusher for pushing each driven portion of the clip feeding ladder member.