An object of the present invention is to provide a method for producing a therapeutic agent for liver diseases containing stromal cells derived from adipose tissue, which is excellent in convenience in preparation of a therapeutic agent while maintaining an excellent therapeutic effect on the stromal cells derived from adipose tissue against liver diseases, and a therapeutic agent for liver diseases obtained by such a producing method. The present invention is directed to a therapeutic agent for liver diseases containing stromal cells derived from adipose tissue which has been subjected to both cryopreservation and thawing at least twice.
