Disclosed herein are an anti-bacterial, anti-fungal and anti-viral composition with excellent sterilizing power, deodorization and adhesion activity, an application thereof, and a method for preparing the same. The composition with sterilizing activity against bacteria, fungus and virus comprises colloidal silver particles in an amount of from 11 wt % to 15 wt %, titanium dioxide nanoparticles in an amount of from 18 wt % to 25 wt %, a dispersion stabilizer in an amount of from 0.01 wt % to 10 wt %, a binder in an amount of from 0.1 wt % to 4 wt %, and a balance of water in an amount required to form 100 wt %.
