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Vectors and methods for the mutagenesis of mammalian genes
专利权人:
George A Gaitanaris
发明人:
George A Gaitanaris
申请号:
US10974603
公开号:
US08389795B2
申请日:
2004.10.27
申请国别(地区):
US
年份:
2013
代理人:
摘要:
Disclosed herein are methods for mutagenizing a mammalian gene, the methods involving introducing into a mammalian cell a retroviral vector which includes a splice acceptor sequence, a transcription termination sequence, and retroviral packaging and integration sequences, the introducing step being carried out under conditions which allow the vector to integrate into the genome of the cell. Also disclosed are retroviral vectors for use in these methods as well as methods for the use of mutagenized cells.
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