INSTITUT PASTEUR;INSTITUT NATIONAL DE LA SANTE ET DE LA RECHERCHE MEDICALE
发明人:
CHARNEAU, PIERRE,ZENNOU, VERONIQUE,PFLUMIO, FRANCOISE,SIRVEN, AUDE,DUBART KUPPERSCHMITT, ANNE
申请号：
CA2382832
公开号：
CA2382832C
申请日:
2000.10.10
申请国别(地区):
CA
年份:
2012
代理人:
摘要：
The present ivention provides nucleic acid, vectors, viruses, and recombinantcells comprising triple-stranded structures, such as those resulting fromcentral initiation and termination of HIV-1 reverse transcription at thecenter of HIV-1 linear DNA genomes. These triplex structures can act as a cis-determinant of HIV-1 DNA nuclear import, allowing infection of non-dividingtarget cells. In one aspect, the presence of the DNA triplex sequence in anHIV vector strongly stimulates gene transfer in hematopoietic stem cells. Theinvention also provides methods of using these triplex structures for makingrecombinant cells, as well as methods of using the recombinant cells toexpress proteins of interest both in vitro and in vivo.
