A suturing assembly including a first puncture element including a sharp distal end for puncturing tissue, a second puncture element including a sharp distal end for puncturing tissue, the distal ends of the first and second puncture elements being spaced from each other by a gap, and a spacer arranged for sliding distally and proximally on at least one of the first and second puncture elements. A suturing assembly including a first puncture element including a sharp distal end for puncturing tissue, a second puncture element including a sharp distal end for puncturing tissue, the distal ends of the first and second puncture elements being spaced from each other by a gap, and a spacer arranged for sliding distally and proximally on at least one of the first and second puncture elements. Methods for identifying and evaluating biochemical entities useful as biomarkers for bladder cancer, target identification/validation, and monitoring of drug efficacy are provided. Also provided are suites of small molecule entities as biomarkers for bladder cancer.Un montaje de sutura incluye un primer elemento de perforación que a su vez incluye un extremo distante filoso para la perforación del tejido, un segundo elemento de perforación que incluye un extremo distante filoso para la perforación del tejido, los extremos distantes del primer y segundo elementos de perforación están separados entre sí por una separación, y un espaciador colocado para deslizarse, en forma distante y próxima, al menos en uno del primer y segundo elementos de perforación.
