Clayton McKee,Christopher J. Murphy,Paul Russell,Shaun P. Garland
申请号：
US13762290
公开号：
US20130211542A1
申请日:
2013.02.07
申请国别(地区):
US
年份:
2013
代理人:
摘要：
The present invention provide methods for preparing nanostructured membranes. The methods include: providing a substrate with a charged silanized surface forming a multilayered membrane containing at least two polyelectrolytes inducing polyelectrolyte phase separation crosslinking the multilayered membrane and covalently linking the multilayered membrane to the silanized surface. Methods for fabricating membrane replicas are also disclosed, as well as devices such as cell- and tissue-culture substrates that contain the membranes and membrane replicas. Resulting materials exhibit topographic features and compliance of the extracellular matrix in vivo.
