XUAN, JIM, W.,CHIN, JOSEPH, L.,PANCHAL, CHANDRA, J.
申请号：
WO2002CA01342
公开号：
WO03018800(A3)
申请日:
2002.08.30
申请国别(地区):
世界知识产权组织国际局
年份:
2003
代理人:
摘要：
The utility of the promoter/enhancer region (i.e. regulatory region) of the PSP94 gene in targeting expression of heterologous genes, to the prostate gland was analyzed. Three breeding lines (i.e., line A, line B and line C) of the PSP-TGMAP model described herein were established with constructs containing regulatory regions with or without additional exon/intron sequence of the PSP94 gene. These regulatory regions were operatively linked to the simian virus 40 (SV40) T antigen (Tag, including both the large T and small t antigens). Histopathology evaluation of tumor grade, transgene expression, tumor responsiveness to androgen deprivation and tumor metastatic potential were analyzed and reported herein. This study indicates that regulatory regions (e.g., the promoter/enhancer with exon/intron region) of the PSP94 gene efficiently target the expression of heterologous genes to prostate tissue. Furthermore, the PSP-TGMAP model provides a valuable tool for the analysis of prostate cancer development and for developing new gene therapy vector for the treatment of prostate cancer. Based on this study, mouse PSP94 promoter/enhancer region may be used in the generation of a "shuttle vector" fit for prostate targeting in both mouse CaP model and in human clinical trial.
