ABSTRACT We propose in this patent application, an improved medium composition which will be useful to grow a poly-bacterial probiotic preparation and assess its antimicrobial potential. The proposed medium can support the normal growth and metabolite production of all the bacteria contained in the probiotic, irrespective of their oxygen requirements, without the use of expensive equipment. After incubation of the probiotic microorganisms, the supernatant containing metabolites can be tested against pathogens of interest for antimicrobial activity. Also, probiotics and pathogens can be cultured in the same medium to study the competitive over-growth of the probiotic over the pathogen. This medium will be useful to assess the validity of claims made by probiotic manufacturers and will also serve as an important tool in probiotic research.
