ISOLATED NUCLEIC ACID MOLECULES, EXPRESSION CASSETTE, METHODS FOR PROTECTION A PLANT OF AN INSECT PREGNANT, METHOD OF OBTAINING A PROCESSED PLANT, ISOLATED PROTOXIN, COMPOSITION, METHOD FOR IMPACTING AN INSECT PRAGUE
E.I. DU PONT DE NEMOURS AND COMPANY;PIONEER HI-BRED INTERNATIONAL, INC.
发明人:
Albert L. Lu,Andre R. Abad,Billy F. McCutchen,Eric J. Schepers,Gary C. Chun,Janet A. Rice,Rafael Herrmann,Ronald D. Flannagan
申请号：
BRPI0611681
公开号：
BRPI0611681B1
申请日:
2006.06.06
申请国别(地区):
BR
年份:
2018
代理人:
摘要：
isolated nucleic acid molecules, methods for protecting a plant from an insect pest, method for obtaining transformed plants, isolated insect protoxin, composition, method for impacting an insect pest, isolated polypeptide showing proteolytic activity. Compositions and methods are provided to protect a plant from an insect pest. nucleic acid molecules encoding insect protoxins or insect toxins are modified to comprise at least one proteolytic activation site that is sensitive to an insect digestive protease. The cleavage of an insect protoxin, modified at the proteolytic activation site by an insect digestive protease, produces an active insect toxin in the insect pest digestive system. Cleavage of a modified insect toxin of the invention into a proteolytic activation site results in the production of an insect toxin active in the insect digestive system which exhibits increased relative pesticidal activity relative to the insect toxin not containing the insect site. proteolytic activation. Methods of using modified insect protoxin and modified insect toxin nuclei and their encoded polypeptides to protect a plant from insect pests are provided. Particular embodiments of the invention further provide insect modified protoxin and insect modified toxin compositions and formulations, expression cassettes, plants, plant cells and transformed seeds. Also disclosed herein are insect digestive proteases and nucleic acid molecules encoding them. Additionally, there is further provided a method for obtaining transformed plant comprising stably transforming a plant cell with at least one polynucleotide construct, cultivating said transformed plant cell under conditions of plant growth and regenerating a transformed plant.moléculas de ácido nucléico isolada, métodos para proteger uma planta de uma praga de inseto, método para obtenção de plantas transformadas, protoxina para inseto isolada, composição, método para impactar uma praga de inseto, polipeptídeo isolado apresentando
