PIONEER HI-BRED INTERNATIONAL, INC.;E.I. DU PONT DE NEMOURS AND COMPANY
发明人:
ABAD, ANDRE,FLANNAGAN, RONALD D.,HERRMANN, RAFAEL,LU, ALBERT,MCCUTCHEN, BILLY F.,PRESNAIL, JAMES K.,RICE, JANET A.,WONG, JAMES F.,YU, CAO-GUO
申请号：
CA2551011
公开号：
CA2551011C
申请日:
2004.12.20
申请国别(地区):
CA
年份:
2013
代理人:
摘要：
Compositions and methods for protecting a plant from an insect pest areprovided. In particular, nucleic acid sequences encoding insect protoxinsmodified to comprise at least one proteolytic activation site that issensitive to a plant protease or an insect gut protease are provided. Cleavageof the modified protoxin at the proteolytic activation site by a proteaseproduces an active insect toxin. Methods of using the modified insect protoxinnucleic acid sequences and the polypeptides they encode to protect a plantfrom an insect pest are provided. Particular embodiments of the inventionfurther provide modified insect protoxin compositions and formulations,expression cassettes, and transformed plants, plant cells, and seeds.
