A sensing blowpipe includes a sensing part, a patterned circuit layer, and a nano-wire in strip shape. The sensing part is both insulating and flexible, and includes a gas flow. The patterned circuit layer is disposed on the sensing part, and includes an electrode pair. The electrode pair includes two electrodes. Each of the electrodes has a tip portion which has a predetermined angle and which is toward the other electrode. The nano-wire connects the tip portions of the electrodes. When a gas flows through the gas flow of the sensing part, the nano-wire contacts the gas. The invention also provides a method of making a sensing blowpipe, and a breath detection system and a method of using the same一種感測吹管,供使用者吹待測氣體,包含感測段、圖案化電路層及條狀的奈米線。感測段以絕緣及具可繞性的材料所製成,並包括供待測氣體流通的氣體流道。圖案化電路層設置於感測段上,並包括一對電極對,電極對具有二間隔相對的電極,每一電極具有成預定夾角且朝向另一電極的尖端部。奈米線連接該電極對之二電極的尖端部,使得電極對的電極藉由奈米線而電連接。當待測氣體流過氣體流道時,奈米線接觸待測氣體。本發明還提供感測吹管的製作方法,及呼氣檢測系統及其使用方法。10‧‧‧感測吹管11‧‧‧感測段12‧‧‧圖案化電路層123‧‧‧尖端部124‧‧‧對外端部