A plant water culture frame and a plant growth ensuring system are disclosed. The plant water culture frame includes a frame body provided with at least one water culture space layer; at least one culture tray provided in the water culture space and used for containing nutrient solution; a plant-fixed basket support component provided in the culture tray and supporting a plant-fixed basket to make it floating with the nutrient solution; and at least one plant-fixed basket provided on the plant-fixed basket support component. The embodiments of the present disclosure effectively avoid having the plant root system submerged when the liquid level of the nutrient solution is too high.